Journal: Surgical Neurology International

Article Title: Poor diagnostic value of isocitrate dehydrogenase 1 R132H immunohistochemistry for determination of isocitrate dehydrogenase 1 status in patients with glioblastoma

doi: 10.25259/SNI_881_2024

Figure Lengend Snippet: Sequencing results of IDH1 mutations and wild-type samples; (left) wild-type and (right) IDH1 R132H. IDH1: Isocitrate dehydrogenase 1. (Right, in blue arrow): IDH1 mutation at R132H

Article Snippet: Following the initial processing step, sections were incubated at room temperature with primary antibodies anti-human IDH1 R132H mutant specific AB (GTX57185 Genetex, IHC 132, mouse monoclonal AB, at 1:200 dilution), followed by 30 min of incubation with the poly horseradish peroxidase (HRP) non-biotin detection system.

Techniques: Sequencing, Mutagenesis

Journal: Surgical Neurology International

Article Title: Poor diagnostic value of isocitrate dehydrogenase 1 R132H immunohistochemistry for determination of isocitrate dehydrogenase 1 status in patients with glioblastoma

doi: 10.25259/SNI_881_2024

Figure Lengend Snippet: IHC results of IDH R132H mutation; (left) positive and (right) negative. IHC: Immunohistochemistry, IDH1: Isocitrate dehydrogenase 1. Magnification: 100x

Article Snippet: Following the initial processing step, sections were incubated at room temperature with primary antibodies anti-human IDH1 R132H mutant specific AB (GTX57185 Genetex, IHC 132, mouse monoclonal AB, at 1:200 dilution), followed by 30 min of incubation with the poly horseradish peroxidase (HRP) non-biotin detection system.

Techniques: Mutagenesis, Immunohistochemistry

Journal: Surgical Neurology International

Article Title: Poor diagnostic value of isocitrate dehydrogenase 1 R132H immunohistochemistry for determination of isocitrate dehydrogenase 1 status in patients with glioblastoma

doi: 10.25259/SNI_881_2024

Figure Lengend Snippet: Result of IDH1 mutation by IHC analysis and direct sequencing method ( n =33).

Article Snippet: Following the initial processing step, sections were incubated at room temperature with primary antibodies anti-human IDH1 R132H mutant specific AB (GTX57185 Genetex, IHC 132, mouse monoclonal AB, at 1:200 dilution), followed by 30 min of incubation with the poly horseradish peroxidase (HRP) non-biotin detection system.

Techniques: Mutagenesis, Sequencing

Journal: Surgical Neurology International

Article Title: Poor diagnostic value of isocitrate dehydrogenase 1 R132H immunohistochemistry for determination of isocitrate dehydrogenase 1 status in patients with glioblastoma

doi: 10.25259/SNI_881_2024

Figure Lengend Snippet: The survival rate of patients with IDH1 status. Patients with IDH1 mutant have a higher survival rate than patients with gliomas with wild-type IDH1 status, whether based on the immunohistochemistry or PCR (Log rank mantle cox, P = 0.0014). IDH1: Isocitrate dehydrogenase 1, PCR: Polymerase chain reaction. WT: Wild type, WT-IHC :Wild type-immunohistochemistry.

Article Snippet: Following the initial processing step, sections were incubated at room temperature with primary antibodies anti-human IDH1 R132H mutant specific AB (GTX57185 Genetex, IHC 132, mouse monoclonal AB, at 1:200 dilution), followed by 30 min of incubation with the poly horseradish peroxidase (HRP) non-biotin detection system.

Techniques: Mutagenesis, Immunohistochemistry, Polymerase Chain Reaction

Journal: Experimental and Therapeutic Medicine

Article Title: Clinical characteristics and prognostic analysis of patients with HIV and glioma: A case series and literature review

doi: 10.3892/etm.2024.12380

Figure Lengend Snippet: Characteristics of HIV-positive patients with brain glial tumors treated at Beijing Ditan Hospital.

Article Snippet: The primary antibodies CD31 (cat. no. ZA-0568), CD68 (cat. no. ZM-0060), CD163 (cat. no. ZM-0428), IDH1 R132H mutation-specific (cat. no. ZM-0447) and Ki-67 (cat. no. ZM-0166) were purchased from ZSGB-BIO (OriGene Technologies, Inc.) and used at a dilution of 1:100.

Techniques: